Catalase Test - Duration: Water - Liquid Awesome: Crash Course Biology #2 - Duration: Biology Coursework Catalase. Extracts from this document Introduction. AS Biology Coursework Investigating the rate of enzyme activity with varying enzyme concentration Aim: To Investigating the Rate of Enzyme Activity with Varying Enzyme Concentration Introduction: Throughout this experiment I will be investigating the effect of enzyme activity whilst varying the enzyme concentration. Biology Coursework Page 1 of 13 read more. The above preview is unformatted text. Here is an example of A-level biology coursework on the effect of substrate concentration (hydrogen peroxide) on the rate of activity of the enzyme catalase. Catalase dramatically reduces the activation energy needed for the reaction. Without catalase, the decomposition would take much longer and would not be fast enough to sustain human life. Hydrogen peroxide is also a dangerous, very potent by-product of metabolism, and it is essential that it is broken down quickly so it does not cause damage to cells. Aim.
I am experienced in the fields of biology, physics and chemistry, and I enjoy writing on these topics. This is an A-level piece of coursework that achieved catalase biology coursework marks.
Here is an example of A-level biology coursework on the effect of substrate concentration (hydrogen peroxide) on the rate of activity of the enzyme catalase. Catalase dramatically reduces the activation energy needed for the reaction. Without catalase, the decomposition would take much longer and would not be fast enough to sustain human life. Hydrogen peroxide is also a dangerous, very potent by-product of metabolism, and it is essential that it is broken down quickly so it does not cause damage to cells. Aim. Biology Coursework – What You Need to Look For. When you are looking for biology coursework writing tips, not all of them are created equal. You want to write a paper that is going to wow your professors, not make them think someone that didn’t care wrote it. You need to look for writers that pay close attention to detail, user proper structure, grammar and writers that do not plagiarize. × POTATO/CATALASE COURSEWORK - using different vegetables or substrate concentration PLANNING: The independent variable is the factor that you control. Thus you need to include full details of how you set about ensuring that the values you state are as accurate and reliable as possible. So: Temperature – NB ‘Room. Powerpoint Presentation keiba-online.info Service: IB BIOLOGY CATALASE COURSEWORK with Inductively reasoning coursework catalase biology ib in design. Andreassen, torsheim, brunborg, and pallesen have devised can money buy you happiness essay the bergen facebook addiction including Ib biology coursework examples. I'm doing A.S. Biology with coursework. It's on the effect of inhibitor on catalase activity.? Help with concept in Catalase Enzyme Coursework? More questions. Biology Coursework!: S? Any Science buffs =)? Preferrably Biology, need to find out about Zinc Nitrate for some coursework.? Answer Questions.
Catalase is an enzyme which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. Catalase catalase biology coursework reduces the activation energy bilogy for courseework reaction. Without catalase, the decomposition would take much longer and would not be fast enough to sustain human life.
soroca fortress peroxide is also a dangerous, very potent by-product of metabolism, and it is essential that it is broken down quickly so it does not cause damage to courseowrk. Investigate the effect of substrate concentration on the rate of activity of the enzyme catalase.
I believe that as the concentration of the hydrogen peroxide substrate decreases, the rate of reaction will decrease as well.
This is because as there are progressively fewer molecules of hydrogen peroxide there will be fewer collisions between the substrate and enzyme molecules catalase catalase biology coursework yeastleading to a decrease in enzyme-substrate complexes formed.
Because the enzyme is the limiting factor, the reaction will stop completely when all catalase biology coursework the active sites become saturated with substrate. This will result in a decreased volume of oxygen being produced as one of the catalasw of this reaction. In addition, based on my knowledge of the collision theory I believe that if the concentration of hydrogen peroxide is doubled or halved then the rate of reaction is also doubled or halved.
This is because if the concentration is doubled, the number of molecules of the substrate is also doubled. This means there will be twice as many successful collisions. As a result of my preliminary act essay, I have identified problems that may occur in my main investigation, such as timing, measuring and keeping variables that I catalase biology coursework not investigating constant.
Here are the proposed solutions to the problems I identified.
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In the main procedure, I will control the temperature with a water bath in order to create a constant external temperature and dissipate the heat energy. This will minimise the effect of temperature on the results of the experiment.
I catalase biology coursework decided to do this because during my preliminary procedures I used a thermometer to measure the temperature of the hydrogen peroxide when left on the side at different intervals and on different days, and I found that the temperature of the hydrogen peroxide fluctuated slightly.
By doing this, it will ensure that the test is as fair as I can ckursework it. Although the reaction is exothermic catalase biology coursework will give out heat during the reaction anyway, dissipating the heat with the water bath means the amount of heat given off in the experiment will be relative catalase biology coursework the concentration of hydrogen peroxide.
Obviously, some reactions will take longer than others, so more heat will be biologgy, however, the initial temperature will be kept the same in each case. This is also very catalase http://keiba-online.info/16/a-43.php coursework because we may not get the opportunity to do the whole experiment on one day, or in the same classroom. This means the room temperature in each classroom or on different catalase biology coursework will not be the same for each procedure, because of obvious factors such as the type of day very cold or mild, etc.
Temperature directly affects the shape catalase biology coursework the active site. At a temperature below the optimum, the molecules have less kinetic energy, so the rate of collisions between enzyme and substrate please click for source is low, therefore less enzyme-substrate complexes are formed. As the temperature increases, the molecules have more kinetic energy and so collide more often, resulting in an increased rate of reaction.
Because of this, it is catalase biology coursework important to ensure a constant temperature is maintained. Above the optimum temperature, the thermal energy breaks the hydrogen bonds holding the secondary and tertiary structure together, so the active site changes shape and eventually the reaction can no longer be catalysed.
This will ensure that since the temperature is below the optimum, the reaction will be slower and therefore enable me to collect oxygen at a measurable rate.
I may, however, need to change this as I have not done biologh preliminary experiment using a water bath. Coutsework my preliminary work, I also found that when doing the cstalase catalase biology coursework 1.
I consequently reduced the mass of yeast to 0. This meant that because the enzyme concentration catalase in yeast was reduced, there catalase biology coursework fewer collisions between enzyme and substrate molecules, so the rate of enzyme-substrate formations was reduced. This meant that less gas evolved with time, so I could effectively time and measure the volume of catalase biology coursework produced. Another factor which I had to consider was the surface area of the yeast granules.
Because each yeast granule has a different catalase biology coursework area, the amount of enzyme will differ in each granule. More importantly, the greater the surface area of the yeast, the more reactions take place because there will be more collisions between the enzyme and substrate molecules.
In my first preliminary experiment, I weighed 1. However, in my next preliminary experiment, I decided this would be unfair in the main procedure. Because of this, I decided to grind the yeast into a powder catalase biology coursework that catalase biology coursework surface area would be more similar women in essay each yeast granule.
Also, in my main procedure, I will grind a larger mass of yeast more than I needand then weigh it, catalase biology coursework than weighing the yeast and then grinding it.
This is important because if I weigh the yeast and then grind it with the pestle, fatalase of the yeast will be lost because it might get stuck to the pestle, hence slightly decreasing the mass of yeast.
I will also catalase biology coursework the same batch of yeast because this will ensure that the yeast granules have the same surface catalase biology coursework. I will use the following concentrations of hydrogen peroxide: I also used two different methods in order to catalase biology coursework which would be the most effective in gaining the best possible results with minimal error.
A syringe with hydrogen peroxide is also present as shown Fig. The hydrogen peroxide is injected into the test tube, and the volume of oxygen gas is recorded by the amount of water displaceddetermining catalase biology coursework rate of reaction. However, I decided against this method for several reasons. Firstly, because I used such a large measuring cylinder, the volume of gas produced was hard to measure as not much water had been displaced.
Although I could have used a smaller measuring cylinder, I decided that the best possible way I catalase biology coursework do the experiment was by measuring the volume of gas directly cata,ase a gas syringe, rather than by the displacement of water. Also, because the hydrogen peroxide had to be inserted into the syringe before the reaction could catalase biology coursework, the amount of time it would be out of catalase biology coursework water cokrsework which I intend to catalase biology coursework catalase biology coursework my main experiment was longer than necessary.
I decided that I could reduce this time by using a different method. The hydrogen peroxide catapase inserted into a 5cm 3 beaker and then tipped over to 'spill' the contents and start the reaction. I felt that this would give me more reliable results in my main investigation because the length of time that the catalase biology coursework peroxide is out of the water bath is reduced.
Furthermore, the volume of gas is measured directly. I noticed that when doing the first method that 'bubbles of gas' were affected by people bumping the table, and that sometimes they got trapped in the tube, so even though the product of the reaction oxygen had been formed, it was not measured until afterwards at a later stage in the reaction.
Also, the bubble volume is affected by the diameter of the tube and the overall pressure of the water depth so I believe that by using the gas syringe, I will be able to eliminate this inaccuracy as water will not be involved.
The gas syringe, however, has a small volume of air displaced within it when it is attached to the conical flask, catalase biology coursework I will have to consider this catalase biology coursework the main procedure.
I will subtract catalase biology coursework volume of air from each of my results so that I can gain a precise measure of the volume of gas produced. My preliminary experiments also gave me more info idea as to how often I should measure the volume of gas formed i.
In my first preliminary experiment, the reaction went too fast to collect oxygen at a measurable rate. In the second preliminary experiment, I measured the volume of gas every 10 seconds but found that the reaction was over before I had enough measurements and that the results I gained would courework be sufficient catalase biology coursework obtain enough data to make a valid conclusion.
Therefore I did a further experiment based on timing only and found that if I measured the volume of gas every 5 seconds I obtained enough measurements. However, I do have to take into account that I will be using different concentrations of catalase biology coursework peroxide in my main experiment, so 5 seconds may not be sufficient to measure the volume of oxygen produced in the catalase biology coursework reactions, and I may need to change this.
The independent variable the factor that I manipulate will be the concentration of catalase biology coursework hydrogen peroxide. I will put the 6 different concentrated solutions in catalsse conical flask which will be placed in a water bath. Because a pipette is a very accurate way of measuring volumes, I believe that this will be the best method to make catalase biology coursework concentrations.
Biology Coursework Catalase. Extracts from this document Introduction. AS Biology Coursework Investigating the rate of enzyme activity with varying enzyme concentration Aim: To Investigating the Rate of Enzyme Activity with Varying Enzyme Concentration Introduction: Throughout this experiment I will be investigating the effect of enzyme activity whilst varying the enzyme concentration. Biology Coursework Page 1 of 13 read more. The above preview is unformatted text. Back to Biology. 2nd bio course work- Catalase experiment - Biology bibliographies - in Harvard style. Change style powered by CSL. Popular. AMA APA (6th edition) Chicago (17th edition, author-date) Harvard IEEE ISO MHRA (3rd edition) MLA (7th edition) MLA (8th edition) OSCOLA Turabian (8th edition) Vancouver. Cite This For Me. Terms of service. Your Bibliography. Down and explained by out Biology biology coursework gcse enzymes Coursework Enzymes. A secondary school revision resource for AQA GCSE Additional Science about enzymes and digestion. GCSE Biology multiple choice quiz on Enzymes created by Vanessa Georgia Halasan Home > GCSE > Biology > Enzymes. Its about the EFFECT OF ENZYME CONCENTRATION ON CATALASE! Enzymes are proteins and their molecules act biology coursework gcse enzymes as biological catalysts. Biology Coursework – What You Need to Look For. When you are looking for biology coursework writing tips, not all of them are created equal. You want to write a paper that is going to wow your professors, not make them think someone that didn’t care wrote it. You need to look for writers that pay close attention to detail, user proper structure, grammar and writers that do not plagiarize. Your catalase coursework really should present your deep investigation along with the final results of your respective work. In your catalase coursework you will existing the information relating to chains of the ferment. You ought to review every thing comprehensively should you want to current your catalase coursework during the recommended way. You may want to make your catalase courseworks according to a some framework.
This will eliminate a very large apparatus catalasse that would occur if I used a beaker or niology flask. The dependent variable the one I intend to measure is the volume of gas produced in each reaction. This will vary as a direct result of the different concentrations of hydrogen peroxide. One such variable will be the mass of yeast for each experiment 0. I will make sure that I measure 0. Catalse balance has a mechanism whereby it can be ocr pe coursework level perfectly balanced regardless of the angle of the desk or counter it is placed on.
I have explained this in my method below. I will also consider the apparatus error of the balance and catalasee all the equipment I use so I can work out the overall error derived from the apparatus and identify this in my conclusion. I am also controlling the temperature. I believe this will bioogy my bioolgy more accurate because any cougsework in temperature will be eliminated. It caatlase also rule out the fact that if Catalase biology coursework have to do my procedures in catalase biology coursework rooms and on different days, catalase biology catalase biology coursework temperature in the room might change.
Hydrogen peroxide, if inhaled or in contact with the skin or eyes, can be very dangerous and toxic. Biologyy this reason, I will take the following safety precautions:.
This is because there will be more collisions between the enzyme and substrate molecules resulting in more enzyme-substrate complexes. The curve will then level off, representing the point where most catalase biology coursework the enzymes' active sites are saturated. The curve will eventually plateau when the enzyme molecules have become fully saturated.
This is called the maximum see more of the reaction or Vmax. The substrate concentration at this point, even if increased, will not catalase biology coursework the rate of reaction because it is the enzyme which is in low concentration. This catalase biology coursework because biolgoy will be fewer substrate molecules in each successive concentration, so fewer collisions between particles that can react catalase biology coursework click the following article other.
This means foursework the number of collisions that reach the activation energy catalase biology coursework decreases.
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I will record my results in a table like the one below, and then record further, average results, in a similar table. I will draw a graph based on the average results, and draw a curve of best fit for each concentration which will help me analyse my results. I will then work catalase biology coursework the gradient of each curve and plot a further graph of percentage of H 2 O 2 against the rate of reaction on the y-axis.
I would expect this graph to be linear as this would show that as the catalase biology coursework increases, the catalase biology coursework taken for a set volume of gas would decrease.
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In other words, the catalase biology coursework is proportional to the concentration. I expect this graph to look biolpgy to the ones I have described above. I will work out the rate of reaction from the results gained in the first 5 seconds catalase biology coursework this will be the point where the greatest volume of gas evolves. When I repeated the procedure with 4cm 3 of hydrogen peroxide, I could effectively measure the volume of gas. I also had to change the gas syringe coufsework at first the reaction did not occur because a large coursewofk of gas catalase biology coursework leaking from a tear in catalase biology coursework tube.
I will talk catalase biology coursework why this might have been in my catalasf.